for assistance call: 573-882-1134

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April 2017

Conference Announcements

14th Congress Advancing Xenotransplantation Science Worldwide


6th Swine in Biomedical Research Conference


November 1, 2016

Updated Publications


October 1, 2016

Publication release

Suggested Guidelines for Tissue Collection

August 1, 2016

Publication release

Birth of potential new model: PAH KO
Donation of a new model: Dr. Monique Lorson, MU donates SMN -/+ animals

February 2016

Publication release

Birth of two potential new models

COL6A

APC

October 2015

Publication release

DNA Methylation supports pig as biomedical model.

September 2015

Publication release

Engineering protein processing of the mammary gland to produce abundant hemophilia B therapy in milk.

Zhao J, Xu W, Ross JW, Walters EM, Butler SP, Whyte JJ, Kelso L, Fatemi M, Vanderslice NC, Giroux K, Spate LD, Samuel MS, Murphy CN, Wells KD, Masiello NC, Prather RS, Velander WH. Engineering protein processing of the mammary gland to produce abundant hemophilia B therapy in milk.

July 2015

Publication release

A Genetic Porcine Model of Cancer

L.B. Schook , T.V. Collares, W. Hu,Y. Liang, F. M. Rodrigues, L. A. Rund, K. M. Schachtschneider, F.K. Seixas, K. Singh, K.D. Wells, E. M. Walters, R.S. Prather, C.M. Counter

Targeted disruption of CD1d prevents NKT cell development in pigs

G. Yang, B. L. Artiaga, T.J. Hackmann, M.S. Samuel, E.M. Walters, S. Salek-Ardakani, J.P. Driver

February 2015

New Strains Added

The NSRRC would like to extend “Thank You” to Dr. Bob Petters from North Carolina State University for his generous donation to the NSRRC. Dr. Petters donated 19 different strains to the NSRRC to be used to study Eye disorders.

November 2014

Publication release

Use of the CRISPR/Cas9 system to produce genetically engineered pigs from in vitro-derived oocytes and embryos

Whitworth KM, Lee K, Benne JA, Beaton BP, Spate LD, Murphy SL, Samuel MS, Mao J, O'Gorman C, Walters EM, Murphy CN, Driver J, Mileham A, McLaren D, Wells KD, Prather RS.

Oct 8, 2014

Press release

NSRRC accepts large donation of Transgenic Swine Eye Models.
More details to come later

May 2014

Publication release

Engraftment of human iPS and allogeneic porcine cells into pigs with inactivated RAG2 and accompanying severe combined immunodeficiency.

Kiho Lee, Deug-Nam Kwon, Toshihiko Ezashia, Yun-Jung Choi, Chankyu Park, Aaron C. Ericsson, Alana N. Brown, Melissa Samuel, Kwang-wook Park, Eric Walters, Dae Young Kim, Jae-Hwan Kim, Craig L. Franklin, Clifton N. Murphy, R. Michael Roberts, Randall S. Prather, and Jin-Hoi Kim

 

Archived News Links

Strain Availability


To request swine or cryopreserved material from the NSRRC, please contact NSRRC@missouri.edu, at (573) 882-1134.

There are a variety of samples available from the strains listed below: A few examples are fetal fibroblast, fresh and cryopreserved semen, live animals, hearts, femurs, kidneys, liver, Day 50 eyes, and Day 60 fetal hearts. Please inquire about the sample request and availability; we should be able to meet your requests in a timely manner. All NSRRC strain distributions require a NSRRC Outgoing Material Transfer Agreement.

List of Available Strains

Stock # Strain Name Background Affected Gene(s) Availability Genomic Alteration
NSRRC:0001 Truline® Hampshire outbred N/A cryopreserved wildtype
NSRRC:0002 Truline® Duroc outbred N/A cryopreserved wildtype
NSRRC:0003 Truline® Landrace outbred N/A cryopreserved wildtype
NSRRC:0004 Truline® Large White outbred N/A cryopreserved wildtype
NSRRC:0005 Minnesota Mini closed population N/A live wildtype
NSRRC:0008 Ossabaw closed population N/A live wildtype
NSRRC:0009

GGTA1 knockout;CD55

closed population CD55 and GGTA1 live GGTA1 knockout and CD55 transgene
NSRRC:0010 GFP NT5 outbred N/A cryopreserved Transgene: CAG promoter driving eGFP
NSRRC:0011 FAT-1 outbred FAT1 cryopreserved Transgene: expression of hFAT1
NSRRC:0012 Yucatan closed population N/A live-limited wildtype
NSRRC:0013 NIH Mini g/g inbred N/A cryopreserved wildtype
NSRRC:0014 NIH Mini c/c inbred N/A live wildtype
NSRRC:0015 NIH Mini a/a inbred N/A cryopreserved wildtype
NSRRC:0016 GFP NT92 outbred Green fluorescent Protein live Transgene: CAG promoter driving the enhanced green fluorescent protein
NSRRC:0017 Rhodopsin NIHc/c RHO live Transgene: Rhodopsin (RHO) P23H substitution
NSRRC:0018 FVIII outbred FVII, FURIN, SERPINA1 cryopreserved Transgenes: Human coagulation factor VIII; Human alpha 1-antitrypsin; Propeptide cleavage enzyme (PACE/FURIN)
NSRRC:0019 FIX outbred FIX, FURIN, VWF cryopreserved Transgenes: Human coagulation factor IX; Human alpha 1-antitrypsin; von Willibrand factor
NSRRC:0020 GFP Yucatan Yucatan Green fluorescent Protein cryopreserved Transgene: CAG promoter driving the enhanced green fluorescent protein
NSRRC:0021 Tie2-eNOS Yucatan NOS3 cryopreserved Transgene: Tie2 promoter driving endothelial Nitrate Oxide Synthase (NOS3)
NSRRC:0022 Tie2-Catalase Yucatan CAT cryopreserved Transgene: Human Catalase (CAT)
NSRRC:0024 GFP-Proteasomes Minnesota Mini PSMA1 live

Transgene: GFP-Proteasome Fusion Protein (PSMA1)

NSRRC:0025 Multi-Xeno outbred GGTA1, CD39, CD55, CD59 cryopreserved

GGTA1 knockout; with three randomly integrated transgenes: human decay-accelerating factor (CD55); CD39; CD59 and thrombomodulin

NSRRC:0026 NLS-CAG-eGFP Minnesota Mini Green Fluorescent Protein cryopreserved

Transgene: CAG promoter driving a Nuclear localized enhanced Green fluorescent Protein

NSRRC:0027 CAG-Tomato Minnesota Mini tdTomato live-limited

Transgene: CAG promoter driving tdTomato

NSRRC:0028 ALP-CD outbred CDA cryopreserved Transgene: Alpha fetoprotein (AFP) promoter driving cytidine deaminase (CDA)
NSRRC:0029 ALB-xTK outbred TK1 cryopreserved

Transgene: Albumin (ALB) promoter driving Thymidine Kinase (TK1)

NSRRC:0030 ZP3-Cre Minnesota Mini ZP3-CRE-CRE live-limited

Transgene: Zona Pecullida 3 (ZP3) promoter driving CRE-eGFP

NSRRC:0031 UBC-Tomato Minnesota Mini tdTomato live-limited

Transgene: Ubiquitin C (UBC) promoter driving tdTomato

NSRRC:0032 NLS-UBC-eGFP Minnesota Mini Green Fluorescent Protein cryopreserved

Transgene: Ubiquitin C (UBC) promoter driving Nuclear Localized eGFP

NSRRC:0033 Onco-pig Minnesota Mini KRASG12D and p53R172H live

Transgene: CAG LoxP Stop driving KRASG12D and p53R172H

NSRRC:0034 DMD Yucatan DMD cryopreserved

Deletion of Exon 42 of DMD

NSRRC:0035 RAG2 KO Minnesota Mini RAG2 live

RAG2 Knockout

NSRRC:0036 BRCA knockdown outbred BRCA1 cryopreserved

Transgene: CSN2 promoter driving micro RNAs directed against BRAC1 and RASSF1

NSRRC:0037 CD55 into GGTA1 Minnesota Mini GGTA1 and CD55 live-limited

GGTA1 knockout with a transgene for human decay-accelerating factor (CD55) inserted into GGTA1

NSRRC:0038 CD1D KO outbred CD1D live

CD1D knockout

NSRRC:0039 FGF8 outbred FGF8 charcterizing

truncated version of FGF8

NSRRC:0040 ISL1-Cre outbred ISL1 charcterizing

Transgene: ISL1 - tissue specific expression of CRE

NSRRC:0041 wild-type- White line outbred N/A cryopreserved

wildtype

NSRRC:0042 Wild-Type-Purple outbred N/A cryopreserved

wildtype

NSRRC:0043 Wild-type-Green outbred N/A cryopreserved

wildtype

NSRRC:0044 Rhodopsin Pro347Leu-Type 1 outbred RHO cryopreserved

Transgene: Pro347Leu in RHO (Type I) in pubs

NSRRC:0045 Rhodopsin Pro347Leu-Type II outbred RHO cryopreserved

Transgene: Pro347Leu in RHO (Type II)

NSRRC:0046 Rhodopsin Pro347Ser outbred RHO cryopreserved

Transgene: Pro347Ser in RHO

NSRRC:0047 ELOVL4- Line 102 outbred ELOVL4 cryopreserved

Transgene: 5 bp deletion in ELOVL4

NSRRC:0048 ELOVL4 Line 912 outbred ELOVL4 cryopreserved

Transgene: ELOVL4 Y270X with the enhanced yellow fluorescent protein

NSRRC:0049 ELOVL4- Line 913 outbred ELOVL4 cryopreserved

Transgene: ELOVL4 Y270X with the enhanced yellow fluorescent protein

NSRRC:0050 ELOVL4- Line 301 outbred ELOVL4 cryopreserved

Transgene: ELOVL4 Y270X with the enhanced yellow fluorescent protein

NSRRC:0051 ELOVL4- Line 303 outbred ELOVL4 cryopreserved

Transgene: ELOVL4 Y270X with the enhanced yellow fluorescent protein

NSRRC:0052 ELOVL4- Line 304 outbred ELOVL4 cryopreserved

Transgene: ELOVL4 Y270X with the enhanced yellow fluorescent protein

NSRRC:0053 PROML1 - Line 001 outbred PROM1 cryopreserved

Transgene: R373C mutation of human PROM1

NSRRC:0054 PROML1- Line 307 outbred PROM1 cryopreserved

Transgene: wild type human PROML1

NSRRC:0055 PROML1- Line 905 outbred PROM1 cryopreserved

Transgene: wild type human PROML1

NSRRC:0056 PROML1- Line 908 outbred PROM1 cryopreserved

Transgene: wild type human PROML1

NSRRC:0057 CTRP outbred C1QTNF5 (CTRP) cryopreserved

Transgene: S163R mutation of pig CTRP (C1QTNF5)

NSRRC:0058 CTRP-Line 2 outbred C1QTNF5 (CTRP) cryopreserved

Transgene: S163R mutation of pig CTRP (C1QTNF5)

NSRRC:0059 Str8 mitochondrial localized eYGFP outbred Enhanced Yellow Fluorescent Protein cryopreserved

Transgene: male germ cell (STR8) tissue specific expression of enhance yellow fluroescent protein (EYFP)

NSRRC:0060 TMPRSS2 outbred TMPRSS2 charcterizing

TMPRSS2 knockout

NSRRC:0061 CD39 and CD55 into GGTA1 Minnesota Mini GGTA1 cryopreserved

GGTA1 knockout with human decay-accelerating factor (CD55) and CD39 transgenes inserted into GGTA1

NSRRC:0062 FAH outbred FAH cryopreserved

FAH Knockout

NSRRC:0063 SMN domestic SMN charcterizing

SMN Knockout

NSRRC:0064 APC Minnesota Mini APC charcterizing

APC Knockout

(Click on the Stock # link for additional information.)

PROCESS: