Donated by Dr. Kiho Lee, University of Missouri.
These pigs were made by zygote injection of the CRISPR CAS-9 system with guides targeting both RAG2 and IL2RG genes. Resulting piglets were RAG2/IL2RG knockouts. These piglets are immunodeficient and present a B-T-NK SCID phenotype.
Publications:
- Hendricks-Wenger A, Aycock KN, Nagai-Singer MA, Coutermarsh-Ott S, Lorenzo MF, Gannon J, Uh K, Farrell K, Beitel-White N, Brock RM, Simon A, Morrison HA, Tuohy J, Clark-Deener S, Vlaisavljevich E, Davalos RV, Lee K, Allen IC. Establishing an immunocompromised porcine model of human cancer for novel therapy development with pancreatic adenocarcinoma and irreversible electroporation. Sci Rep. 2021 Apr 7;11(1):7584. doi: 10.1038/s41598-021-87228-5. PMID: 33828203; PMCID: PMC8027815.
- Imran KM, Gannon J, Morrison HA, Tupik JD, Tintera B, Nagai-Singer MA, Ivester H, Madanick JM, Hendricks-Wenger A, Uh K, Luyimbazi DT, Edwards M, Coutermarsh-Ott S, Eden K, Byron C, Clark-Deener S, Lee K, Vlaisavljevich E, Allen IC. Successful In Situ Targeting of Pancreatic Tumors in a Novel Orthotopic Porcine Model Using Histotripsy. Ultrasound Med Biol. 2023 Nov;49(11):2361-2370. doi: 10.1016/j.ultrasmedbio.2023.07.013. Epub 2023 Aug 16. PMID: 37596154; PMCID: PMC10529075.
- Lei S, Ryu J, Wen K, Twitchell E, Bui T, Ramesh A, Weiss M, Li G, Samuel H, Clark-Deener S, Jiang X, Lee K, Yuan L. Increased and prolonged human norovirus infection in RAG2/IL2RG deficient gnotobiotic pigs with severe combined immunodeficiency. Sci Rep. 2016 Apr 27;6:25222. doi: 10.1038/srep25222. PMID: 27118081; PMCID: PMC4846862.