Whitworth KM, Benne JA, Spate LD, Murphy SL, Samuel MS, Murphy CN, Richt JA, Walters E, Prather RS, Wells KD. Zygote injection of CRISPR/Cas9 RNA successfully modifies the target gene without delaying blastocyst development or altering the sex ratio in pigs. Transgenic Res. 2016 Oct 15. PMID: 27744533
Choi YJ, Lee K, Park WJ, Kwon DN, Park C, Do JT, Song H, Cho SK, Park KW, Brown AN, Samuel MS, Murphy CN, Prather RS, Kim JH. Partial loss of interleukin 2 receptor gamma function in pigs provides mechanistic insights for the study of human immunodeficiency syndrome. Onco target. 2016 Jul 24. PMID: 27463006
Lee W, Miyagawa Y, Long C, Ekser B, Walters E, Ramsoondar J, Ayares D, Tector AJ, Cooper DK, Hara H. Expression of NeuGc on Pig Corneas and Its Potential Significance in Pig Corneal Xenotransplantation. Cornea. 2016 Jan;35(1):105-13. PMID: 26418433
Whitworth KM, Mao J, Lee K, Spollen WG, Samuel MS, Walters EM, Spate LD, Prather RS. Transcriptome Analysis of Pig In Vivo, In Vitro-Fertilized, and Nuclear Transfer Blastocyst-Stage Embryos Treated with Histone Deacetylase Inhibitors Postfusion and Activation Reveals Changes in the Lysosomal Pathway. Cell Reprogram. 2015 Aug;17(4):243-58. PMID: 26731590
Redel BK, Prather RS. Meganucleases Revolutionize the Production of Genetically Engineered Pigs for the Study of Human Diseases. Toxicol Pathol. 2016 Apr;44(3):428-33. PMID: 26516165
Zhao J, Xu W, Ross JW, Walters EM, Butler SP, Whyte JJ, Kelso L, Fatemi M, Vanderslice NC, Giroux K, Spate LD, Samuel MS, Murphy CN, Wells KD, Masiello NC, Prather RS, Velander WH. Engineering protein processing of the mammary gland to produce abundant hemophilia B therapy in milk. Sci Rep. 2015 Sep 21;5:14176. PMID: 26387706
Beaton BP, Kwon DN, Choi YJ, Kim JH, Samuel MS, Benne JA, Wells KD, Lee K, Kim JH, Prather RS. Inclusion of homologous DNA in nuclease-mediated gene targeting facilitates a higher incidence of bi-allelically modified cells. Xenotransplantation. 2015 Sep-Oct;22(5):379-90. PMID: 26381494
Yang G, Artiaga BL, Hackmann TJ, Samuel MS, Walters EM, Salek-Ardakani S, Driver JP. Targeted disruption of CD1d prevents NKT cell development in pigs. Mamm Genome. 2015 Jun;26(5-6):264-70. PMID: 25930071
Men H, Spate LD, Murphy CN, Prather RS. Cryopreservation of In Vitro-Produced Early-Stage Porcine Embryos in a Closed System. Biores Open Access. 2015 May 1;4(1):258-65. PMID: 26309801
Lee K, Davis A, Zhang L, Ryu J, Spate LD, Park KW, Samuel MS, Walters EM, Murphy CN, Machaty Z, Prather RS. Pig oocyte activation using a Zn²? chelator, TPEN. Theriogenology. 2015 Oct 1;84(6):1024-32. PMID: 26143360
Schook LB, Collares TV, Hu W, Liang Y, Rodrigues FM, Rund LA, Schachtschneider KM, Seixas FK, Singh K, Wells KD, Walters EM, Prather RS, Counter CM. A Genetic Porcine Model of Cancer. PLoS One. 2015 Jul 1;10(7) PMID: 26132737
Park J, Lai L, Samuel MS, Wax D, Prather RS, Tian X. Disruption of Mitochondrion-To-Nucleus Interaction in Deceased Cloned Piglets. PLoS One. 2015 Jun 11;10(6) PMID: 26067091
Huang J, Zhang H, Wang X, Dobbs KB, Yao J, Qin G, Whitworth K, Walters EM, Prather RS, Zhao J. Impairment of preimplantation porcine embryo development by histone demethylase KDM5B knockdown through disturbance of bivalent H3K4me3-H3K27me3 modifications. Biol Reprod. 2015 Mar;92(3):72. PMID: 25609834
Mao J, Zhao MT, Whitworth KM, Spate LD, Walters EM, O’Gorman C, Lee K, Samuel MS, Murphy CN, Wells K, Rivera RM, Prather RS. Oxamflatin treatment enhances cloned porcine embryo development and nuclear reprogramming. Cell Reprogram. 2015 Feb;17(1):28-40. PMID: 25548976
Li G, Jia Q, Zhao J, Li X, Yu M, Samuel MS, Zhao S, Prather RS, Li C. Dysregulation of genome-wide gene expression and DNA methylation in abnormal cloned piglets. BMC Genomics. 2014 Sep 24;15:811. PMID: 25253444
Whitworth KM, Lee K, Benne JA, Beaton BP, Spate LD, Murphy SL, Samuel MS, Mao J, O’Gorman C, Walters EM, Murphy CN, Driver J, Mileham A, McLaren D, Wells KD, Prather RS. Use of the CRISPR/Cas9 system to produce genetically engineered pigs from in vitro-derived oocytes and embryos. Biol Reprod. 2014 Sep;91(3):78. PMID: 25100712
Lee K, Kwon DN, Ezashi T, Choi YJ, Park C, Ericsson AC, Brown AN, Samuel MS, Park KW, Walters EM, Kim DY, Kim JH, Franklin CL, Murphy CN, Roberts RM, Prather RS, Kim JH. Engraftment of human iPS cells and allogeneic porcine cells into pigs with inactivated RAG2 and accompanying severe combined immunodeficiency. Proc Natl Acad Sci U S A. 2014 May 20;111(20):7260-5. PMID: 24799706
Walters EM, Prather RS. Advancing swine models for human health and diseases. Mo Med. 2013 May-Jun;110(3):212-5. PMID: 23829105
Spate LD, Murphy CN, Prather RS. High-throughput cryopreservation of in vivo-derived swine embryos. PLoS One. 2013 Jun 7;8(6):e65545. PMID: 23762391
Kwon DN, Lee K, Kang MJ, Choi YJ, Park C, Whyte JJ, Brown AN, Kim JH, Samuel M, Mao J, Park KW, Murphy CN, Prather RS, Kim JH. Production of biallelic CMP-Neu5Ac hydroxylase knock-out pigs. Sci Rep. 2013;3:1981. PMID: 23760311
Isom SC, Stevens JR, Li R, Spollen WG, Cox L, Spate LD, Murphy CN, Prather RS. Transcriptional profiling by RNA-Seq of peri-attachment porcine embryos generated by a variety of assisted reproductive technologies. Physiol Genomics. 2013 Jul 15;45(14):577-89. PMID: 23695885.
EM Walters, RS Prather. Advancing swine models for human health and diseases. Mo Med. 2013 May-Jun;110(3):212-5. PMID: 23829105.
LD Spate, CN Murphy, RS Prather. High-throughput cryopreservation of in vivo-derived swine embryos. PLoS One. 2013 Jun 7;8(6):e65545. doi: 10.1371/journal.pone.0065545. Print 2013.PMID: 23762391.
EL Miles, C O’Gorman, J Zhao, MS Samuel, EM Walters, YJ Yi, M Sutovsky, RS Prather, KD Wells, P Sutovsky.Transgenic pig carrying green fluorescent proteasomes. Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6334-9. doi: 10.1073/pnas.1220910110. Epub 2013 Apr 2. PMID: 23550158.
K Lee, R.S. Prather. 2013. Cloning pigs by somatic cell nuclear transfer. In: Principles of Cloning, 2nd edition (J. Cibelli, J. Gurdon, I. Wilmut, R. Jaenisch, R. Lanza, M. West, K. Campbell, eds). Academic Press (in press).
RS Prather, M. Lorson, J.W. Ross, J. Whyte, E. Walters. 2013. Genetically engineered pig models for human diseases. In: Annual Review of Animal Biosciences, Vol. 1 (L. Andersson, D.E. Bauman, W.C. Brown, H.A. Lewin, S.J. O’Brien, E.A. Ostrander, R.M. Roberts, L.J. Saif, G.E. Seidel, Jr., A. Xu, Eds.). Annual Reviews, Palo Alto, CA. 203-219.
RS Prather. 2013. Pig genomics for biomedicine. Nat Biotechnol. 31(2):122-4. PMID: 23392511 K Lee, BK Redel, L Spate, J Teson, AN Brown, KW Park, E Walters, M Samuel, CN Murphy, RS Prather. 2013. Piglets produced from cloned blastocysts cultured in vitro with GM-CSF. Mol Reprod Dev. 80(2):145-54. PMID: 23239239.
EM Walters, E Wolf, JJ Whyte, J Mao, S Renner, H Nagashima, E Kobayashi, J Zhao, KD Wells, JK Critser, LK Riley, RS Prather. 2012. Completion of the swine genome will simplify the production of swine as a large animal biomedical model. BMC Med Genomics. 5:55. PMID: 23151353.
X Yang, J Mao, EM Walters, M Zhao, J Teson, K Lee, RS Prather. 2012. Xenopus Egg Extract Treatment Reduced Global DNA Methylation of Donor Cells and Enhanced Somatic Cell Nuclear Transfer Embryo Development in Pigs. Biores Open Access. 1(2):79-87. PMID 23515109.
J Mao, K Tessanne, KM Whitworth, LD Spate, EM Walters, MS Samuel, CN Murphy, L Tracy, J Zhao, RS Prather. 2012. Effects of combined treatment of MG132 and scriptaid on early and term development of porcine somatic cell nuclear transfer embryos. Cell Reprogram. 14(5):385-9. PMID: 22917492.
H. Men, EM Walters, H Nagashima, RS Prather. 2012. Emerging applications of sperm, embryo and somatic cell cryopreservation in maintenance, relocation and rederivation of swine genetics. Theriogenology. Nov;78(8):1720-9. PMID: 22898022.
J Mao, KM Whitworth, LD Spate, EM Walters, J Zhao, RS Prather. 2012. Regulation of oocyte mitochondrial DNA copy number by follicular fluid, EGF, and neuregulin 1 during in vitro maturation affects embryo development in pigs. Theriogenology. 78(4):887-97. PMID:22626782.
JW Ross, JP Fernandez de Castro, J Zhao, M Samuel, EM Walters, C Rios, P Bray-Ward, BW Jones, RE Marc, W Wang, L Zhou, J Noel, M McCall, P DeMarco, RS. Prather, H J. Kaplan. Generation of an inbred miniature pig model of retinitis pigmentosa. 2012. Investigative Ophthalmology & Visual Science. 2012. IVOS. 53(1):501-507. PMID: 22247487.
EM Walters, Y Agca, V Ganjam, T Evans. Animal Models Got you Puzzled? Think Pig. 2011. Annuals of The New York Academy of Sciences. PMID: 22211982.
Richards MP, Kathirvel P, Gong Y, Lopez-Hernandez A, Walters EM, Prather RS. Long chain omega-3 fatty acid levels in loin muscle from transgenic (fat-1 gene) pigs and effects on lipid oxidation during storage. 2011. Food Biotechnology 25:103-114.
Hongsheng Men, Chongbei Zhao, Si Wei, Clifton N. Murphy, Martha W. Bennett, August Rieke, Lee Spate, Yang Liu, Eric M. Walters, Melissa S. Samuel, Randall S. Prather, John K. Critser. Birth of piglets from in vitro produced, zona-intact porcine embryos vitrified in a closed system. 2011. 76(2)280-9. Theriogenology. PMID 21458047.
Whyte, J.J., R.S. Prather. 2011. Genetic modifications of pigs for medicine and agriculture. Molecular Reproduction and Development 78:879-891. NIHMSID #299529.
Whyte, J.J., R.S. Prather. 2012. Zinc-finger nucleases to create custom-designed modifications in the swine (sus scrofa) genome. J. Animal Science (accepted 10/24/2011). PMID 22038991.
Whyte JJ, Samuel MS, Mahan E, Padilla J, Simmons GH, Arce-Esquivel AA, Bender SB, Whitworth KM, Hao YH, Murphy CN, Walters, EM, Prather, RS, and Laughlin, MH. 2011. Vascular endothelium-specific overexpression of human catalase in cloned pigs. Transgenic Res. [epub ahead of print].
Zhao J, Whyte JJ, and Prather RS. 2010. Effect of epigenetic regulation during swine embryogenesis and on cloning by nuclear transfer. Cell and tissue research 341:13-21. PMID: 20563602.
Men H, Zhao C, Wei S, Murphy CN, Spate L, Liu Y, Walters EM, Samuel MS, Prather RS, Critser JK. Birth of piglets from in vitro-produced, zona-intact porcine embryos vitrified in a closed system. Theriogenology. 2011 Mar 30.
Whyte JJ, Zhao J, Wells KD, Samuel MS, Whitworth KM, Walters EM, Laughlin MH, and Prather RS. 2011a. Gene targeting with zinc finger nucleases to produce cloned eGFP knockout pigs. Mol. Reprod. Dev. 78:2. PMID 21268178.
Zhao, J., Y. Hao, J.W. Ross, L.D. Spate, E.M. Walters, M.S. Samuel, A. Rieke, C.N. Murphy,R.S. Prather 2010. Histone deacetylase inhibitors improve in vitro and in vivo developmental competence of somatic nuclei nuclear transfer porcine embryos. Cellular Reprogramming 12:75-83. PMCID: PMC2842950.
Ross, J.W., J.J. Whyte, J. Zhao, M. Samuel, K.D. Wells, R.S. Prather. 2010. Optimization of square-wave electroporation for transfection of porcine fetal fibroblasts. Transgenic Research 19:611-620. PMCID: PMC2894267.
Whitworth, K.M., R.S. Prather. 2010. Somatic cell nuclear transfer efficiency- Remodeling and reprogramming: How can it be improved? Molecular Reproduction & Development 77:1001-1015. PMID 20931660.
X Yang, J Mao, EM Walters, M Zhao, K Lee, RS Prather. 2012. Treatment of donor cells with Xenopus oocyte extract enhanced SCNT embryo development. 38th Annual International Embryo Transfer Society. Phoenix, AZ.
J Mao, KM Whitworth, LD Spate, EM Walters, J Zhao, RS Prather. 2012. Supplementation of maturation medium with follicular fluid, Epidermal Growth Factor, and Neuregulin affects mitochondrial DNA replication, oocyte maturation, and embryo development in pigs. 38th Annual International Embryo Transfer Society. Phoenix, AZ.
EM. Walters, MS. Samuel KD Wells, LK Riley, Randall S Prather. 2012. The Pig as a Biomedical Model-How the National Swine Resource and Research Center Can Help You. 38th Annual International Embryo Transfer Society. Phoenix, AZ. PMID:22394972
E. M. Walters, T.J Evans, Y. Agca, V.K. Ganjam. 2011. Development of a Porcine Model for Exposure to Endocrine Disrupting Chemicals. Animal Models and Their Value in Predicting Drug Efficacy and Toxicity. New York Academy of Sciences. New York City, New York.
Prather, R.S. 2011. Technical blocks to efficient pig cloning. CTS-IXA 2011 Joint International Congress, Miami, FL. Oct. 23-26. Whyte, J.J., J. Zhao, K.D. Wells, M.S. Samuel, K.M. Whitworth, E.M. Walters, M.H. Laughlin, R.S. Prather. 2011. Zinc-finger nucleases: new innovations in custom-designed modification of the swine genome. Swine in Biomedical Research Conference July 17-19, Chicago, IL. P 27.
Beaton, B.P., E. Walters, R.S. Prather, K. Wells. 2011. Alpha-Galactosyltransferase (a1,3-Gal) Knockout Genetically Linked to Xenograft Transgenes. Swine in Biomedical Research Conference July 17-19, Chicago, IL. P 28.
Li, C., C. O’Gorman, R.S. Prather, J.A. Green, K.D. Wells. 2011. BWS symptoms in cloned piglets are associated with hypomethylation at the KCNQ-OT1 CpG island. Swine in Biomedical Research Conference July 17-19, Chicago, IL. P 35.
O’Gorman, C.W.. J. Zhao, M.S. Samuel, E.M. Walters, R.S. Prather, P. Sutovsky, M. Sutovsky, K.D. Wells. 2011.Swine in Biomedical Research Conference July 17-19, Chicago, IL. P 36.
Zhao, J.G., E.M. Walters, J. Calcaterra, L. Kelso, K. Giroux, M.S. Samuel, S. Butler, W.H. Velander, R.S. Prather. 2011. Increased propeptide processing of Factor IX in the milk of transgenic pigs by co-expression of Furin. Swine in Biomedical Research Conference July 17-19, Chicago, IL. P 41.
Whyte, J.J., R.S. Prather. 2011. Zinc-Finger Nucleases: New Innovations in Custom-Designed Modification of the Swine Genome. ADSA-ASAS Annual Meeting.
Whitworth, K. J. Teson, K. Lee, J. Mao, K. Tessanne, L.D. Spate, R.S. Prather. 2012. The histone deacetylase inhibitor 4-iodo-Suberoylanilide Hydroxamic Acid (I-SAHA) improves total cell number in pig nuclear transfer blastocysts. International Embryo Transfer Society Annual Meeting. Reprod. Fertil. & Devel. 24: 125.
Tessanne, K., B. Bauer, K. Whitworth, L. Spate, A. Brown and R.S. Prather. 2012. Characterization of the PRMT-DDAH-NO axis during porcine embryo development International Embryo Transfer Society Annual Meeting. Reprod. Fertil. & Devel. 24:185.
JJ Whyte, SC Isom, WG Spollen, SM Blake, EM Walters, RS Prather. 2011. DNA methylome analysis in nuclear transfer cells and its relationship to cloning efficiency in swine. 37th Annual International Embryo Transfer Society. Orlando, FL.
C. O’Gorman, J. Zhao, M Samuel, E. Walters, R. Prather, P. Sutovsky, M. Sutovsky, K.D. Wells.2011. Construction of a genetically engineered pig expressing GFP-labeled proteasomes. 37th Annual International Embryo Transfer Society. Orlando, FL.