To request swine or cryopreserved material from the NSRRC, please contact NSRRC@missouri.edu, at (573) 882-1134.
There are a variety of samples available from the strains listed below: A few examples are fetal fibroblast, fresh and cryopreserved semen, live animals, hearts, femurs, kidneys, liver, Day 50 eyes, and Day 60 fetal hearts. Please inquire about the sample request and availability; we should be able to meet your requests in a timely manner. All NSRRC strain distributions require an NSRRC Outgoing Material Transfer Agreement.
List of Available Strains
| Stock # | Strain Name | Background | Affected Gene(s) | Availability | Genomic Alteration |
|---|---|---|---|---|---|
| NSRRC:0001 | Truline® Hampshire | outbred | N/A | Cryopreserved | wildtype |
| NSRRC:0002 | Truline® Duroc | outbred | N/A | Cryopreserved | wildtype |
| NSRRC:0003 | Truline® Landrace | outbred | N/A | Cryopreserved | wildtype |
| NSRRC:0004 | Truline® Large White | outbred | N/A | Cryopreserved | wildtype |
| NSRRC:0005 | Minnesota Mini | closed population | N/A | Live-limited | wildtype |
| NSRRC:0008 | Ossabaw | closed population | N/A | Currently unavailable | wildtype |
| NSRRC:0009 | GGTA1 knockout; CD55 | closed population | CD55 and GGTA1 | Live | GGTA1 knockout and CD55 transgene |
| NSRRC:0010 | GFP NT5 | outbred | N/A | Cryopreserved | Transgene: CMV promoter driving eGFP |
| NSRRC:0011 | FAT1 | outbred | FAT1 | Cryopreserved | Transgene: expression of hFAT1 |
| NSRRC:0012 | Yucatan | closed population | N/A | Cryopreserved | wildtype |
| NSRRC:0013 | NIH Mini g/g | inbred | N/A | Cryopreserved | wildtype |
| NSRRC:0014 | NIH Mini c/c | inbred | N/A | Live-Very limited | wildtype |
| NSRRC:0015 | NIH Mini a/a | inbred | N/A | Cryopreserved | wildtype |
| NSRRC:0016 | GFP NT92 | outbred | Green fluorescent Protein | Live | Transgene: CAG promoter driving the enhanced green fluorescent protein |
| NSRRC:0017 | Rhodopsin | NIHc/c | RHO | Live | Transgene: Rhodopsin (RHO) P23H substitution |
| NSRRC:0018 | FVIII | outbred | FVII, FURIN, SERPINA1 | Cryopreserved | Transgenes: Human coagulation factor VIII; Human alpha 1-antitrypsin; Propeptide cleavage enzyme (PACE/FURIN) |
| NSRRC:0019 | FIX | outbred | FIX, FURIN, VWF | Cryopreserved | Transgenes: Human coagulation factor IX; Human alpha 1-antitrypsin; von Willibrand factor |
| NSRRC:0020 | GFP Yucatan | Yucatan | Green fluorescent Protein | Cryopreserved | Transgene: CAG promoter driving the enhanced green fluorescent protein |
| NSRRC:0021 | Tie2-eNOS | Yucatan | NOS3 | Cryopreserved | Transgene: Tie2 promoter driving endothelial Nitrate Oxide Synthase (NOS3) |
| NSRRC:0022 | Tie2-Catalase | Yucatan | CAT | Cryopreserved | Transgene: Human Catalase (CAT) |
| NSRRC:0024 | GFP-Proteasomes | Minnesota Mini | PSMA1 | Cryopreserved | Transgene: GFP-Proteasome Fusion Protein (PSMA1) |
| NSRRC:0025 | Multi-Xeno | outbred | GGTA1, CD39, CD55, CD59 | Cryopreserved | GGTA1 knockout; with three randomly integrated transgenes: human decay-accelerating factor (CD55); CD39; CD59 and thrombomodulin |
| NSRRC:0026 | NLS-CAG-eGFP | Minnesota Mini | Green Fluorescent Protein | Cryopreserved | Transgene: CAG promoter driving a Nuclear localized enhanced Green Fluorescent Protein |
| NSRRC:0027 | CAG-Tomato | Minnesota Mini | tdTomato | Cryopreserved | Transgene: CAG promoter driving tdTomato |
| NSRRC:0028 | ALP-CD | outbred | CDA | Cryopreserved | Transgene: Alpha fetoprotein (AFP) promoter driving cytidine deaminase (CDA) |
| NSRRC:0029 | ALB-xTK | outbred | TK1 | Cryopreserved | Transgene: Albumin (ALB) promoter driving Thymidine Kinase (TK1) |
| NSRRC:0030 | ZP3-Cre | Minnesota Mini | ZP3-CRE-GFP | Cryopreserved | Transgene: Zona Pecullida 3 (ZP3) promoter driving CRE-eGFP |
| NSRRC:0031 | UBC-Tomato | Minnesota Mini and Minnesota Mini/Domestic | tdTomato | Live-limited (MN/Domestic)
Cryopreserved (MN Mini) |
Transgene: Ubiquitin C (UBC) promoter driving tdTomato |
| NSRRC:0032 | NLS-UBC-eGFP | Minnesota Mini | Green Fluorescent Protein | Cryopreserved | Transgene: Ubiquitin C (UBC) promoter driving Nuclear Localized eGFP |
| NSRRC:0033 | Onco-pig | Minnesota Mini and Minnesota Mini/Domestic | KRASG12D and p53R172H | Live-limited (MN/Domestic)
Cryopreserved (MN Mini) |
Transgene: CAG LoxP Stop driving KRASG12D and p53R172H |
| NSRRC:0034 | DMD | Yucatan | DMD | Cryopreserved | Deletion of Exon 42 of DMD |
| NSRRC:0035 | RAG2 KO | Minnesota Mini | RAG2 | Live-limited (MN Mini)
Cryopreserved (MN mini/Domestic) |
RAG2 Knockout |
| NSRRC:0036 | BRCA knockdown | outbred | BRCA1 | Cryopreserved | Transgene: CSN2 promoter driving micro RNAs directed against BRAC1 and RASSF1 |
| NSRRC:0037 | CD55 into GGTA1 | Minnesota Mini and Minnesota Mini/Domestic | GGTA1 and CD55 | Live-limited (MN/Domestic)
Cryopreserved (MN Mini) |
GGTA1 knockout with a transgene for human decay-accelerating factor (CD55) inserted into GGTA1 |
| NSRRC:0038 | CD1D KO | outbred | CD1D | Live | CD1D knockout |
| NSRRC:0041 | wild-type- White line | outbred | N/A | Cryopreserved | wildtype |
| NSRRC:0042 | Wild-Type-Purple | outbred | N/A | Cryopreserved | wildtype |
| NSRRC:0043 | Wild-type-Green | outbred | N/A | Cryopreserved | wildtype |
| NSRRC:0049 | ELOVL4-Line 913 | outbred | ELOVL4 | Cryopreserved | Transgene: ELOVL4 Y270X with the enhanced yellow fluorescent protein |
| NSRRC:0050 | ELOVL4-Line 301 | outbred | ELOVL4 | Cryopreserved | Transgene: ELOVL4 Y270X with the enhanced yellow fluorescent protein |
| NSRRC:0052 | ELOVL4-Line 304 | outbred | ELOVL4 | Cryopreserved | Transgene: ELOVL4 Y270X with the enhanced yellow fluorescent protein |
| NSRRC:0057 | CTRP | outbred | C1QTNF5 (CTRP) | Cryopreserved | Transgene: S163R mutation of pig CTRP (C1QTNF5) |
| NSRRC:0058 | CTRP-Line 2 | outbred | C1QTNF5 (CTRP) | Cryopreserved | Transgene: S163R mutation of pig CTRP (C1QTNF5) |
| NSRRC:0059 | Str8 mitochondrial localized eYGFP | outbred | Enhanced Yellow Fluorescent Protein | Cryopreserved | Transgene: male germ cell (STR8) tissue-specific expression of enhancing yellow fluorescent protein (EYFP) |
| NSRRC:0060 | TMPRSS2 | outbred | TMPRSS2 | Characterizing | TMPRSS2 knockout via zygote injection of the CRISPR Cas-9 system. |
| NSRRC:0061 | CD39 and CD55 into GGTA1 | Minnesota Mini | GGTA1 | Cryopreserved | GGTA1 knockout with human decay-accelerating factor (CD55) and CD39 transgenes inserted into GGTA1 |
| NSRRC:0062 | FAH | outbred | FAH | Cryopreserved | FAH Knockout via zygote injection of the CRISPR Cas-9 system. |
| NSRRC:0063 | SMN | domestic | SMN | Characterizing | SMN Knockout |
| NSRRC:0064 | APC | Minnesota Mini | APC | Cryopreserved | Heterozygous modification of the Adenomatous polyposis coli (APC) gene to induce colorectal polyps |
| NSRRC:0069 | SIGLEC1/CD169 |
domestic | SIGLEC1 |
Cryopreserved cells and semen | Insertion of a transgene harboring neomycin resistance into the SIGLEC1 gene through homologous recombination, knocking out gene function. Obtained through somatic cell nuclear transfer and propagated by breeding. Homozygous and heterozygous available. |
| NSRRC:0070 | ANPEP |
domestic | ANPEP |
Cryopreserved cells and semen | Knockout of gene function via zygote injection of the CRISPR Cas-9 system. Homozygous and heterozygous available. |
| NSRRC:0071 | CD163 |
domestic | CD163 |
Cryopreserved cells and semen | Knockout of gene function via zygote injection of the CRISPR Cas-9 system. Homozygous and heterozygous available. |
| NSRRC:0072 | ANTXR1 |
domestic | ANTXR1 |
Cryopreserved | Monoallelic in-frame mutation via zygote injection of the CRISPR Cas-9 system. Available in semen and fetal fibroblast. |
| NSRRC:0075 | FANCA |
domestic | FANCA |
Live | KO of FANCA Exon 4 via zygote injection of the CRISPR Cas-9 system. |
| NSRRC:0076 | FANCD2 |
domestic | FANCD2 |
Cryopreserved cells and semen | Monoallelic KO of FANCD2 via zygote injection of the CRISPR Cas-9 system. A biallelic modification is embryonic lethal. |
| NSRRC:0077 | USH2A |
domestic | USH2A |
Cryopreserved cells | Both biallelic and monoallelic knockout of the USH2A gene via zygote injections of the CRISPR Cas-9 system. This induces Usher Syndrome Type 2. Available in fetal fibroblast. |
| NSRRC:0079 | CLCA1 |
domestic | CLCA1 |
Live | KO of the CLCA1 gene via zygote injections of the CRISPR Cas-9 system, to reduce mucus production in the lungs. |
| NSRRC:0081 | SAP130 |
domestic | SAP130 |
Cryopreserved cells | Monoallelic knockout of the SAP130 gene via zygote injections of the CRISPR Cas-9 system. A biallelic modification is embryonic lethal. Available in fetal fibroblast. |
| NSRRC:0085 | FAH/RAG2 |
domestic | FAH, RAG2 |
Cryopreserved cells | A biallelic knockout of both FAH and RAG2 genes, using the CRISPR Cas-9 system in zygote injections and electroporation. |
| NSRRC:0086 | GGTA1 KO/B4GALNT2 KO/B4GALNT2L KO with CD55 | domestic | GGTA1, B4GALNT2, B4GALNT2L | Live-limited; also available by SCNT | GGTA1 KO/B4GALNT2 KO/B4GALNT2L KO with a transgene for human decay-accelerating factor (CD55) |
| NSRRC:0093 | APP | domestic | APP |
Live-limited | Alzheimers model |
| NSRRC:0094 | PSEN1 | domestic | PSEN1 | Live-limited | Alzheimers model |
| NSRRC:0095 | RAG2/IL2RG | domestic | RAG2, IL2RG |
Cryopreserved | Skid model |
| NSRRC:0096 | IgH (Ig Heavy Chain) |
domestic | IgH |
Cryopreserved | |
| NSRRC:0097 | Large white x Landrace |
domestic | N/A | Cryopreserved | Wildtype, non-modified pigs. |
| NSRRC:0098 | GDF9 | domestic | GDF9 | Live, cryopreserved cells, semen | Modification of the GDF9 gene via zygote injection of the CRISPR Cas-9 system. Resulting phenotype is a lack of folliculogenesis. |
(Click on the Stock # link for additional information.)
PROCESS:
Receiving Investigator must:
Fill out and submit an On-Line Receiving Investigator Application Form.
Provide a Material Transfer Agreement (MTA) using the Institution MTA or the Governmental MTA form.
The NSRRC will then:
Obtain authorization from the Receiving Investigator’s Institution for animal shipment.
Ship animals to the Receiving Investigator’s Institution.
Bill the account. See the Distribution Fees Page for pricing.